Descripció del contingut i pla d'activitats:
Obtención de oxidoreductasa y lipooxigenasa para su utilización en catálisis enzimáticas controladas de ácidos grasos poliinsaturados
Overview (resum en anglès):
The objective of the project is to convert fatty acids into their corresponding aldehydes using a
process known as biocatalysis. Specifically, octanoic and decanoic acid into octanal and decanal.
The proposed strategy is to initially screen commercial enzymes, and see if any of them are
successful in the transformations we want to do.
Carboxylic acid reductase enzymes (CAR) are multi-domain proteins capable of catalyzing the
reaction mentioned, depending on the concentration of ATP and NADPH, of a wide range of fatty
acids in the corresponding aldehydes. This particular reaction is of considerable biotechnological
interest.
The main strategy would be to test commercially available CAR (carboxylic acid reductase) type
enzymes on a laboratory scale to see how well they do the desired transformations. In the future,
depending on the results, the scale-up phase from laboratory to industry would be considered.
As it will be explained in the results below, the enzymes found commercially available do have the
expected effect in our reaction. Therefore, they have a biocatalytic potential in the reaction
explained and applied in the project, therefore they can be employed for obtaining octanal and
decanal.