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Projecte llegit

Títol: Biodisponibilitat de quelados de Gly


Estudiant que ha llegit aquest projecte:


Tutor/Cotutor: SEPULCRE SANCHEZ, FRANCISCO LUIS

Departament: DEAB

Títol: Biodisponibilitat de quelados de Gly

Data inici oferta: 14-06-2022      Data finalització oferta: 14-02-2023


Estudis d'assignació del projecte:
    GR ENG SIS BIOLÒGICS

Lloc de realització:
EEABB

Segon tutor extern: Ghadeer Mattar

Paraules clau:
Quelados de minerales, Glicina, Biodisponibilidad

Descripció del contingut i pla d'activitats:
Estudios de biodisponibilidad, simulando un proceso digestivo, de quelados de diferentes cationes Fe and Zn), obtenidos a diferentes valores de pH y relación molar.

Las muestras se analizan mediante absorción atómica

Overview (resum en anglès): The aim of this work is the study of the bioavailability of glycine (Gly)-iron (Fe) chelates to determine which combination of Gly:Fe and pH is more bioavailable to the human organism, as well as to establish whether Gly chelates are more bioavailable in water than ferrous sulphate, the most widely used inorganic salt in iron supplementation. For this purpose, 21 samples of chelates with different Gly:Fe ratios were analysed at three different pHs: acid pH (0.9), initial pH (4) and isoelectric point pH (6). For the bioavailability study, an in vitro system was used to simulate human digestion consisting of a two-step sample digestion process: peptic digestion and continuous flow dialysis. Continuous flow dialysis required the preparation of a NaHCO3 solution with a specific concentration for each sample, which was determined by an acidity titration of the sample after peptic digestion. The peptic digestion and dialysis procedure was performed in triplicate for each sample, obtaining a total of 63 replicates. For the determination of iron bioavailability, the iron in the sample was quantified before and after undergoing the dialysis process to evaluate the percentage of iron that had been absorbed. The results determined that the most optimal combination of pH and Gly:Fe ratio, i.e., the one with the highest bioavailability in water, is the 1:1 ratio at pH 6, pH at which glycine is at its isoelectric point. On the other hand, a comparison was also carried out between the bioavailability of iron sulphate and that of the chelates studied, concluding that, in water, ferrous sulphate has a higher bioavailability than Gly chelates.

KEY WORDS: Mineral chelates, Glycine, Bioavailability.



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