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Projecte llegit

Títol: Response of different strains of the unicellular microalgae Chlamydomonas reinhardtii to photoperiod and light intensity

Estudiant que ha llegit aquest projecte:

Tutor/a o Cotutor/a: ROIG VILLANOVA, IRMA

Departament: DEAB

Títol: Response of different strains of the unicellular microalgae Chlamydomonas reinhardtii to photoperiod and light intensity

Data inici oferta: 23-01-2024      Data finalització oferta: 23-09-2024

Estudis d'assignació del projecte:
    GR ENG SIS BIOLÒG 23

Lloc de realització:


En empresa (cal signar un conveni de cooperació)

        Tutor/a Extern: Elena Monte
        Institució/Empresa: Centre de Recerca en Agrigenòmica

Paraules clau:
Chalmydomonas, wild-type, photoperios, light intensity

Descripció del contingut i pla d'activitats:
1-Caracterització de la resposta a fotoperíode i llum de diferents soques de la microalga unicel·lular Chlamydomonas reinhardtii. S'estudiarà diferents paràmetres fisiològics i de creixement en fotoperíodes de diferents llargades (dia curt, 12:12 i dia llarg), així com en diferents tipus de qualitat de llum (blanca, blava, vermella) i d'intensitat de llum.
2-Familiarització amb el procediment de generació de mutants en Chlamydomonas reinhardtii mitjançant CRISPR.
3-Genotipat dels possibles mutants de Chlamydomonas obtinguts per CRISPR.
4-Seqüenciació dels mutants de Chlamydomonas obtinguts per CRISPR.

Overview (resum en anglès): Chlamydomonas reinhardtii is a model organism of which many different strains are used widely in research. However, these strains have not been highly characterized, leaving knowledge gaps of their specific traits.
This study aims to understand how different light regimes affect these microalgae's growth and photosynthetic efficiency. Additionally, it also evaluates the effect of a change in light intensity for the strains. This is done by evaluating the impact of various photoperiods and light intensities on different wild-type strains: CLip, 4A+, CC-124, and CC-125. Specifically, it examines pigment quantification, cell size, the maximum quantum yield of Photosystem II (Fv/Fm) as an indicator of the efficiency of the photosynthetic apparatus, and non-photochemical quenching (NPQ) which evaluates the capacity of cells to dissipate the excess of absorbed light energy.
The results obtained indicate that the photoperiod and light intensity have an effect on the parameters evaluated. For all the strains, chlorophyll content decreases in continuous light conditions and carotenoid content is only affected by photoperiod. The cell size of all strains varies with both strain and photoperiod. Light intensity increases cell size in CC-125, and CC-124 is affected by the combination of photoperiod and light intensity. The photosynthetic efficiency and photoprotective mechanisms are affected by photoperiods and high light for all the strains. Altogether, this characterization will help select the more appropriate ones for specific studies in the future.

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