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Projecte llegit

Títol: Evaluation of tomato ¿1-Pyrroline-5-Carboxylate Synthetase (P5CS) variabiity under drought stress

Estudiant que ha llegit aquest projecte:

Tutor/a o Cotutor/a: MAS SERRA, MAITE

Departament: DEAB

Títol: Evaluation of tomato ¿1-Pyrroline-5-Carboxylate Synthetase (P5CS) variabiity under drought stress

Data inici oferta: 21-02-2025      Data finalització oferta: 21-02-2025

Estudis d'assignació del projecte:
    GR ENG SIS BIOLÒG 23

Lloc de realització:
ERASMUS

Segon tutor/a extern: Ali Ahmad Naz.Osnabrück University

Paraules clau:
Tomaquera, estrès, genòmica, al·lels, prolina

Descripció del contingut i pla d'activitats:
Serà necessari fer una immersió en la bibliografia i en les bases de dades que, fins al moment, hi hagi publicades i accessibles sobre el tema, o sigui, buscar informació i llegir tota la que es trobi. També caldrà aprendre tots els protocols necessaris per realitzar les diverses tasques previstes. La durada mínima de realització del treball és de dos mesos i mig.

Overview (resum en anglès): Tomato (Solanum lycopersicum L.) is the most produced vegetable worldwide. Tomato breeding has been extensively studied to improve cultivation efficiency and stress resilience. From its origin to domestication, tomato underwent a severe genetic bottleneck that greatly reduced its genetic variability, including alleles that confer tolerance to abiotic stresses. This project evaluates the potential effects of Solanum pennellii wild alleles of the ¿1-Pyrroline-5-Carboxylate Synthetase (P5CS) gene on drought stress response in cultivated tomato. Developmental and biochemical traits (plant height, growth, number of leaves per plant, relative chlorophyll content (SPAD values), and proline accumulation) were measured in three accessions: the cultivated variety M82, and two introgression lines (IL 6-1 and IL 8-1) carrying wild P5CS alleles. Plants were grown under controlled conditions until 35 days after sowing; then the control group was watered daily, and the stress group received no water during the whole drought treatment (7 days). No relevant differences were observed between introgression lines and M82 in any of the evaluated traits under drought stress. Additionally, no correlation was found between proline concentration and plant height under either treatment condition. These results indicate that introgression of these wild P5CS alleles alone does not enhance drought resistance in cultivated tomato. Therefore, proline accumulation appears not to be the primary factor underlying drought tolerance in wild tomatoes, and these specific alleles may not be adequate targets for breeding drought-resistant cultivars.

Aquest projecte està relacionat amb l'adaptació al Canvi Climatic? Sí

Aquest projecte està relacionat amb la digitalització del seu àmbit de treball? No

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