CBL - Campus del Baix Llobregat

Projecte llegit

Títol: Verificación de los métodos moleculares para la detección de Salmonella spp. y Listeria monocytogenes mediante PCR en matrices alimentarias

Estudiant que ha llegit aquest projecte:

Tutor/a o Cotutor/a: BLANCO ABELLAN, MONICA

Departament: MAT

Títol: Verificación de los métodos moleculares para la detección de Salmonella spp. y Listeria monocytogenes mediante PCR en matrices alimentarias

Data inici oferta: 08-09-2021      Data finalització oferta: 08-04-2022

Estudis d'assignació del projecte:
    GR ENG ALIMENT 2016

Lloc de realització:


En empresa (cal signar un conveni de cooperació)

        Tutor/a Extern: Irene Mir
        Institució/Empresa: BIOSER S. A.

Segon tutor/a extern: Esther Bermejo

Paraules clau:
Verificación, Validación, Salmonela, Listeria monocytogenes, ISO y PCR

Descripció del contingut i pla d'activitats:
El trabajo consiste en realizar un estudio basado en rtPCR (real time PCR) y la posterior verificación mediante un estudio estadístico de dos microorganismos patógenos de la industria alimentaria como son la Salmonella y la Listeria monocytogenes en muestras de clientes o en su defecto en matrices alimentarias las cuales consumimos en nuestro dia a dia.

Overview (resum en anglès): The polymerase chain reaction (PCR) is a molecular method born in 1983 with several uses in different fields of study, such as archaeology, medicine, and food safety among others.

That is why the work is focused on the use of this molecular method to make a comparison between kits for the detection of pathogenic microorganisms such as Salmonella and Listeria monocytogenes in different food matrices. This will be possible thanks to a previous inoculation at different concentrations. We even wanted to go further by simulating a verification of the validation of the kits used in the study based on the ISO 16140:3 standard.

In order to do this, four foods from four different categories (a dairy product, an egg product, a vegetable and a fish) and three different detection kits were chosen. These three kits will be compared with each other and the PCR results obtained will be analysed and commented. To perform the simulation of the verification, the detection limit will be calculated considering the PCR results obtained knowing that all the test samples have been inoculated with the analysed microorganisms.

The results obtained have not been entirely conclusive due to small limitations that have been observed throughout the development of the experimental work, such as, for example, cross-contamination. Despite this, an analysis of the PCR results obtained has been carried out and the results obtained have been accepted as valid. This has allowed us to correctly calculate and comment on the detection limits obtained.

Among the most relevant conclusions we can highlight that the work should be repeated in order to obtain conclusive and significant results that would give rise to develop a statistical study through them and to be able to discern which of the three kits is the optimal one to perform the verification many factors must be taken into account, so there is no single valid answer. Furthermore, the detection limits obtained are considerably low considering that most of them have detected the lowest inoculation performed in the different matrices.


© CBLTIC Campus del Baix Llobregat - UPC