Projecte matriculat

Títol: Proving the effect of intramitochondrial enzyme filamentation on mitochondrial morphology and cell fitness

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Tutor/a o Cotutor/a: SEPULCRE SANCHEZ, FRANCISCO LUIS

Departament: DEAB

Títol: Proving the effect of intramitochondrial enzyme filamentation on mitochondrial morphology and cell fitness

Data inici oferta: 02-07-2024      Data finalització oferta: 02-03-2025

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Estudis d'assignació del projecte:

GR ENG SIS BIOLÒG 23

Lloc de realització:


En empresa (cal signar un conveni de cooperació)

        Tutor/a Extern: Héctor García Seisdedos
        Institució/Empresa: Inst. de Biologia Molecular BCN (CSIC)

Paraules clau:
Filament-forming proteins; mitochondrial morphology

Descripció del contingut i pla d'activitats:
We demonstrated that proteins evolve on the edge of supramolecular assembly, and that symmetric homo-oligomeric complexes are particularly prone to self-assembly into filaments.

Such reversible filamentation of homo-oligomeric complexes has also been reported for natural, wild-type complexes, particularly for metabolic enzymes during cell stress and starvation. In eukaryotes, homo-oligomeric complexes with the potential to form filaments tend to be mitochondrial proteins. However, the consequences of intramitochondrial enzyme filamentation for mitochondrial morphology and function, as well as overall cellular fitness, have not been investigated.

To address this open question, we aim to investigate the effects of filament-forming proteins targeted to mitochondria in living yeast cells. These proteins are tagged with fluorescent proteins, they are foreign to yeast cells, and they are enzymatically inactive, ensuring that any observed effects can be attributed solely to the filamentation of the complex within the yeast mitochondria. We will characterise the mitochondrial morphology of cells expressing the filament-forming complexes using fluorescence microscopy and compare it to cells expressing a version of the complex that is unable to form filaments. Additionally, we will test the fitness effects of enzyme filamentation within the mitochondria using growth curves and/or competition assays.

Taken together, by targeting filament-forming proteins to mitochondria in living yeast cells and analysing their effects on mitochondrial morphology and cellular fitness, this project will provide insights into the consequences of intramitochondrial enzyme filamentation.

Orientació a l'estudiant: Treball experimental. Coneixement de tècniques de biologia molecular i de bioquímica (cultiu de llevats, expressió i purificació de proteïnes...)

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